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Retention of endogenous growth factors in the Thyroid Acellular Scaffold (TAS). The bar chart shows the concentrations (pg/mL) of key growth factors—Vascular Endothelial Growth Factor (VEGF), Transforming Growth Factor-β (TGF-β), Hepatocyte Growth Factor (HGF), <t>Fibroblast</t> Growth Factor (FGF), Epidermal Growth Factor (EGF), and Platelet-Derived Growth Factor (PDGF)—in native thyroid tissue and the TAS, as quantified by ELISA. Although the decellularization process resulted in a significant reduction in the concentration of all measured growth factors, the TAS successfully retained a substantial amount of these bioactive molecules. Data are presented as mean ± standard deviation. **p < 0.01 compared to native tissue.
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Retention of endogenous growth factors in the Thyroid Acellular Scaffold (TAS). The bar chart shows the concentrations (pg/mL) of key growth factors—Vascular Endothelial Growth Factor (VEGF), Transforming Growth Factor-β (TGF-β), Hepatocyte Growth Factor (HGF), Fibroblast Growth Factor (FGF), Epidermal Growth Factor (EGF), and Platelet-Derived Growth Factor (PDGF)—in native thyroid tissue and the TAS, as quantified by ELISA. Although the decellularization process resulted in a significant reduction in the concentration of all measured growth factors, the TAS successfully retained a substantial amount of these bioactive molecules. Data are presented as mean ± standard deviation. **p < 0.01 compared to native tissue.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Development of a biomimetic thyroid acellular scaffold as a 3D platform for modeling thyroid cancer aggressiveness and drug resistance

doi: 10.3389/fbioe.2025.1692549

Figure Lengend Snippet: Retention of endogenous growth factors in the Thyroid Acellular Scaffold (TAS). The bar chart shows the concentrations (pg/mL) of key growth factors—Vascular Endothelial Growth Factor (VEGF), Transforming Growth Factor-β (TGF-β), Hepatocyte Growth Factor (HGF), Fibroblast Growth Factor (FGF), Epidermal Growth Factor (EGF), and Platelet-Derived Growth Factor (PDGF)—in native thyroid tissue and the TAS, as quantified by ELISA. Although the decellularization process resulted in a significant reduction in the concentration of all measured growth factors, the TAS successfully retained a substantial amount of these bioactive molecules. Data are presented as mean ± standard deviation. **p < 0.01 compared to native tissue.

Article Snippet: Specific ELISA kits were used to detect the content of vascular endothelial growth factor (VEGF, R&D Systems, RRV00), transforming growth factor-β (TGF-β, R&D Systems, RTB100B), hepatocyte growth factor (HGF, R&D Systems, MHG00), fibroblast growth factor (FGF, R&D Systems, MFB00), epidermal growth factor (EGF, R&D Systems, DY3214), and platelet-derived growth factor (PDGF, R&D Systems, MBB00).

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation